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Countercurrent
Chromatography (CCC) and Partition Chromatography are Countercurrent Separation (CCS) Techniques
Inspired by Craig's countercurrent
distribution (CCD),
the two most-practived modern countercurrent separation (CCS) techniques are (high-speed) countercurren chromatography ([HS]CCC) and partition chromatography
(CPC).
Owing to the fact that well-established experimental and instrumental setups exist for HSCCC
and CPC
that do not use true countercurrent flow of liquids, partition chromatography
is the more generic term and actually describes the underlying principle
of partitioning
the analytes between two immiscible liquid phases.
The immense separation power of
CCC/CPC results from the following unique poperties:
-
As only solvents are involved and solid supports
are avoided, irreversible adsorption cannot occur,
sample degradation is minimized, and a theoretical
100% sample recovery is achievable.
-
The partition coefficient, K, is an intrinsic physicochemical constant of each molecular species, which can be used to target CCS analysis for individual compounds
Countercurrent Research at UIC Chicago Our natural products group at UIC has an extensive track record and expertise in applying and developing CCC experimental techniques. Countercurrent Chromatography: Background Countercurrent Chromatography: Resources
- Applications: compound classes, GUESS and solvent system selection, separation modes, modelling, and chiral CCC/CPC
- Support & Guidelines : general guidelines, modes & types of CCC experiments, instrument types, manufacturers and distributors, and links
- Literature: general literature, research articles, and literature trends
- Countercurrent Separation review articles covering CCC, CPC, HSCCC, and CCS:
- Introductory materials on CCC
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